Project sanitarium:playing tuberculosis to its end game

Interdisciplinary and collaborative projects between industry and academia provide exceptional opportunities for learning. Project Sanitarium is a serious game for Windows PC and Tablet which aims to embed learning about tuberculosis (TB) through the player taking on the role of a doctor and solving cases across the globe. The project developed as a collaboration between staff and undergraduate students at the School of Arts, Media and Computer Games at Abertay University working with academics and researchers from the Infection Group at the University of St Andrews. The project also engaged industry partners Microsoft and DeltaDNA. The project aimed to educate students through a workplace simulation pedagogical model, encourage public engagement at events and through news coverage and lastly to prototype whether games could be used to simulate a virtual clinical trial. The project was embedded in the Abertay undergraduate programme where students are presented with real world problems to solve through design and technology. The result was a serious game prototype that utilized game design techniques and technology to demystify and educate players about the diagnosis and treatment of one of the world’s oldest and deadliest diseases, TB. Project Sanitarium aims to not only educate the player, but allows the player to become a part of a simulated drug trial that could potentially help create new treatments in the fight against TB. The game incorporates a mathematical model that is based on data from real-world drug trials. The interdisciplinary pedagogical model provides undergraduates with workplace simulation, wider industry collaboration and access to academic expertise to solve challenging and complex problems

Modelling the sun's small scale global photospheric magnetic field

We present a new model for the Sun's global photospheric magnetic field during a deep minimum of activity, in which no active regions emerge. The emergence and subsequent evolution of small- scale magnetic features across the full solar surface is simulated, subject to the influence of a global supergranular flow pattern. Visually, the resulting simulated magnetograms reproduce the typical structure and scale observed in quiet Sun magnetograms. Quantitatively, the simulation quickly reaches a steady state, resulting in a mean field and flux distribution that are in good agreement with those determined from observations. A potential coronal magnetic field is extrapolated from the simulated full Sun magnetograms, to consider the implications of such a quiet photospheric magnetic field on the corona and inner heliosphere. The bulk of the coronal magnetic field closes very low down, in short connections between small-scale features in the simulated magnetic network. Just 0.1% of the photospheric magnetic flux is found to be open at 2:5 Rʘ, around 10 - 100 times less than that determined for typical HMI synoptic map observations. If such conditions were to exist on the Sun, this would lead to a significantly weaker interplanetary magnetic field than is presently observed, and hence a much higher cosmic ray flux at Earth

rLOAD: does sex mediate the effect of acute antiplatelet loading on stroke outcome.

BackgroundBiologic sex can influence response to pharmacologic therapy. The purpose of this proof-of-concept study was to evaluate the medicating effects of estrogen in the efficacy of acute antiplatelet loading therapy on stroke outcome in the rabbit small clot embolic model.MethodsFemale and male (20/group) New Zealand White rabbits were embolized to produce embolic stroke by injecting small blood clots into the middle cerebral artery via an internal carotid artery catheter. Two hours after embolization, rabbits were treated with standard dose antiplatelet loading (aspirin 10 mg/kg plus clopidogrel 10 mg/kg). Primary outcome measures were platelet inhibition, behavioral outcome P 50 (the weight of microclots (mg) that produces neurologic dysfunction in 50% of a group of animals), and effect of endogenous estrogen on outcome.ResultsFor the first time in a non-rodent model of stroke, it was found that higher endogenous estrogen levels resulted in significantly better behavioral outcome in female subjects (r s -0.70, p < 0.011). Platelet inhibition in response to collagen, arachidonic acid, and adenosine diphosphate (ADP) was not significantly different in females with higher vs. lower estrogen levels.ConclusionsBehavioral outcomes are improved with females with higher endogenous estrogen levels treated with standard dose antiplatelet loading. This is the first non-rodent study to demonstrate that higher endogenous estrogen levels in female rabbits appear to be neuroprotective in ischemic stroke. This research supports the further study of the effect of endogenous estrogen levels on outcome with standard dose antiplatelet loading in stroke patients not eligible for revascularization therapies

Pre-capacity building in loosely-coupled collaborations: Setting the stage for future initiatives

This article examines the benefits and limitations of ‘loosely-coupled’ research collaborations between university faculty and 12 grassroots community-based organisations (CBOs). The authors assert that community-based research projects that develop the knowledge base within CBOs, and can be described as ‘pre-capacity building’ work, can be an important stepping stone to the subsequent development of more formal and strategic capacity-building partnership ventures. However, such projects must be approached carefully with a clear understanding of the ‘threshold dimensions’ that must be met before proceeding with any collaboration. Written as a cautionary tale, the authors identify some of the problems that arise when the threshold stage is poorly executed, and more generally speak to the dangers of initiating even loosely-coupled collaborations in the absence of an explicit and well-established campus commitment to and support for community engagement and partnerships. Keywords: Community capacity-building, community-university partnerships, community research, collaboratio

Corticosteroids for presumed pneumocystis pneumonia in patients with HIV infection

Adjunctive corticosteroids decrease mortality in patients with HIV infection who have moderate to severe hypoxemia and suspected or confirmed pneumocystis pneumonia. Corticosteroids lead to a higher incidence of herpetic lesions, but not other opportunistic conditions. (Strength of recommendation: A, based on a systematic review of randomized controlled trials [RCTs].

Tenotomy-induced muscle atrophy is sex-specific and independent of NFκB

The nuclear factor-κB (NFκB) pathway is a major thoroughfare for skeletal muscle atrophy and is driven by diverse stimuli. Targeted inhibition of NFκB through its canonical mediator IKKβ effectively mitigates loss of muscle mass across many conditions, from denervation to unloading to cancer. In this study, we used gain- and loss-of-function mouse models to examine the role of NFκB in muscle atrophy following rotator cuff tenotomy - a model of chronic rotator cuff tear. IKKβ was knocked down or constitutively activated in muscle-specific inducible transgenic mice to elicit a twofold gain or loss of NFκB signaling. Surprisingly, neither knockdown of IKKβ nor overexpression of caIKKβ significantly altered the loss of muscle mass following tenotomy. This finding was consistent across measures of morphological adaptation (fiber cross-sectional area, fiber length, fiber number), tissue pathology (fibrosis and fatty infiltration), and intracellular signaling (ubiquitin-proteasome, autophagy). Intriguingly, late-stage tenotomy-induced atrophy was exacerbated in male mice compared with female mice. This sex specificity was driven by ongoing decreases in fiber cross-sectional area, which paralleled the accumulation of large autophagic vesicles in male, but not female muscle. These findings suggest that tenotomy-induced atrophy is not dependent on NFκB and instead may be regulated by autophagy in a sex-specific manner

A Near-half-century Simulation of the Solar Corona

We present an overview of results from a magnetofrictional model of the entire solar corona over a period of 47 yr. The simulation self-consistently reproduces decades of solar phenomena, varying in duration between rapid eruptions and the long-term solar cycles, from an input of observed active regions emerging at the photosphere. We have developed a geometric approach to use magnetic helicity to identify and localize the frequent eruptions that occur in the simulation. This method allows us to match our results to extreme-ultraviolet observations of transient events. We have analyzed the evolving magnetic topology by computing the squashing factor and segmenting the corona into discrete magnetic domains bounded by the Separatrix-Web. The simulations show a more dynamic structure to the Separatrix-Web than is predicted by potential field models, which may explain solar wind observations

Investigation of the middle corona with SWAP and a data-driven non-potential coronal magnetic field model

The large field-of-view of the Sun Watcher using Active Pixel System detector and Image Processing (SWAP) instrument onboard the PRoject for Onboard Autonomy 2 (PROBA2) spacecraft provides a unique opportunity to study extended coronal structures observed in the EUV in conjunction with global coronal magnetic field simulations. A global non-potential magnetic field model is used to simulate the evolution of the global corona from 1 September 2014 to 31 March 2015, driven by newly emerging bipolar active regions determined from Helioseismic and Magnetic Imager (HMI) magnetograms. We compare the large-scale structure of the simulated magnetic field with structures seen off-limb in SWAP EUV observations. In particular, we investigate how successful the model is in reproducing regions of closed and open structures, the scale of structures, and compare the evolution of a coronal fan observed over several rotations. The model is found to accurately reproduce observed large-scale, off-limb structures. When discrepancies do arise they mainly occur off the east solar limb due to active regions emerging on the far side of the Sun, which cannot be incorporated into the model until they are observed on the Earth-facing side. When such “late” active region emergences are incorporated into the model, we find that the simulated corona self-corrects within a few days, so that simulated structures off the west limb more closely match what is observed. Where the model is less successful, we consider how this may be addressed, through model developments or additional observational products

A statistical comparison of EUV brightenings observed by SO/EUI with simulated brightenings in nonpotential simulations

Open access funding provided by Swiss Federal Institute of Technology Zurich. L.H. and K.B. are grateful to the SNF for the funding of the project number 200021_188390. D.H.M. would like to thank the STFC for support via consolidated grant ST/W001195/1. K.A.M. would like to thank the STFC for support via consortium grant ST/W001098/1.The High Resolution Imager (HRIEUV) telescope of the Extreme Ultraviolet Imager (EUI) instrument onboard Solar Orbiter has observed EUV brightenings, so-called campfires, as fine-scale structures at coronal temperatures. The goal of this paper is to compare the basic geometrical (size, orientation) and physical (intensity, lifetime) properties of the EUV brightenings with regions of energy dissipation in a nonpotential coronal magnetic-field simulation. In the simulation, HMI line-of-sight magnetograms are used as input to drive the evolution of solar coronal magnetic fields and energy dissipation. We applied an automatic EUV-brightening detection method to EUV images obtained on 30 May 2020 by the HRIEUV telescope. We applied the same detection method to the simulated energy dissipation maps from the nonpotential simulation to detect simulated brightenings. We detected EUV brightenings with a density of 1.41×10−3 brightenings/Mm2 in the EUI observations and simulated brightenings between 2.76×10−2 – 4.14×10−2 brightenings/Mm2 in the simulation, for the same time range. Although significantly more brightenings were produced in the simulations, the results show similar distributions of the key geometrical and physical properties of the observed and simulated brightenings. We conclude that the nonpotential simulation can successfully reproduce statistically the characteristic properties of the EUV brightenings (typically with more than 85% similarity); only the duration of the events is significantly different between observations and simulation. Further investigations based on high-cadence and high-resolution magnetograms from Solar Orbiter are under consideration to improve the agreement between observation and simulation.Publisher PDFPeer reviewe

Rapid Start-up and Loading of an Attached Growth, Simultaneous Nitrification/Denitrification Membrane Aerated Bioreactor

Membrane aerated bioreactors (MABR) are attached-growth biological systems used for simultaneous nitrification and denitrification to reclaim water from waste. This design is an innovative approach to common terrestrial wastewater treatments for nitrogen and carbon removal and implementing a biologically-based water treatment system for long-duration human exploration is an attractive, low energy alternative to physiochemical processes. Two obstacles to implementing such a system are (1) the "start-up" duration from inoculation to steady-state operations and (2) the amount of surface area needed for the biological activity to occur. The Advanced Water Recovery Systems (AWRS) team at JSC explored these two issues through two tests; a rapid inoculation study and a wastewater loading study. Results from these tests demonstrate that the duration from inoculation to steady state can be reduced to under two weeks, and that despite low ammonium removal rates, the MABRs are oversized